Prevalence and molecular characterisation of extended-spectrum β-lactamases genes in Escherichia coli and Salmonella spp. isolated from fish samples in Chhattisgarh, India

coli and Salmonella spp. Out of 200 samples collected from retail markets Durg, Raipur, Rajnandgaon Balod districts Chhattisgarh State, 76 (38) 46 (23%) were found contaminated with E. spp., respectively. Multiple antibiotic resistant (MAR) index varied between 0.00 to 0.88 0.11 for isolates, The isolates screened phenotypically ESBL production multiplex PCR targeting blaTEM, blaSHV, andblaCTX-M genes, was carried out followed by sequencing. Prevalence blaSHV blaCTX-M genes in be 13.2, 1.3, 0 6.5, 0, 4.4%, Higher percentage detected plasmid DNA comparison genomic DNA. Sequencing amplicons blaTEM revealed the presence blaTEM-116 blaCTX-M-15 variants, study indicates multidrug producing fish samples, which may pose threat consumers. Keywords: blaCTX-M, coli, Fish, MAR index,